Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.
Identifieur interne : 002684 ( Main/Exploration ); précédent : 002683; suivant : 002685Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.
Auteurs : Yuanzhen Lin [République populaire de Chine] ; Shanzhi Lin ; Hai Guo ; Zhiyi Zhang ; Xiaoyang ChenSource :
- Biotechnology letters [ 1573-6776 ] ; 2013.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Analyse de profil d'expression de gènes (MeSH), Basse température (MeSH), Expression des gènes (MeSH), Feuilles de plante (composition chimique), Glucose 6-phosphate dehydrogenase (génétique), Glucose 6-phosphate dehydrogenase (métabolisme), Malonaldéhyde (analyse), Peroxydes (analyse), Phylogenèse (MeSH), Populus (enzymologie), Populus (génétique), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Stress physiologique (MeSH), Superoxide dismutase (analyse), Tabac (effets des radiations), Tabac (enzymologie), Tabac (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- analyse : Malonaldéhyde, Peroxydes, Superoxide dismutase.
- composition chimique : Feuilles de plante.
- effets des radiations : Tabac.
- enzymologie : Populus, Tabac.
- génétique : Glucose 6-phosphate dehydrogenase, Populus, Protéines recombinantes, Tabac.
- métabolisme : Glucose 6-phosphate dehydrogenase, Protéines recombinantes.
- Alignement de séquences, Analyse de profil d'expression de gènes, Basse température, Expression des gènes, Phylogenèse, Stress physiologique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Cold Temperature (MeSH), Gene Expression (MeSH), Gene Expression Profiling (MeSH), Glucosephosphate Dehydrogenase (genetics), Glucosephosphate Dehydrogenase (metabolism), Malondialdehyde (analysis), Peroxides (analysis), Phylogeny (MeSH), Plant Leaves (chemistry), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sequence Alignment (MeSH), Stress, Physiological (MeSH), Superoxide Dismutase (analysis), Tobacco (enzymology), Tobacco (genetics), Tobacco (radiation effects).
- MESH :
- chemical , analysis : Malondialdehyde, Peroxides, Superoxide Dismutase.
- chemical , genetics : Glucosephosphate Dehydrogenase, Recombinant Proteins.
- chemical , metabolism : Glucosephosphate Dehydrogenase, Recombinant Proteins.
- chemistry : Plant Leaves.
- enzymology : Populus, Tobacco.
- genetics : Populus, Tobacco.
- radiation effects : Tobacco.
- Cold Temperature, Gene Expression, Gene Expression Profiling, Phylogeny, Plants, Genetically Modified, Sequence Alignment, Stress, Physiological.
Abstract
A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress.
DOI: 10.1007/s10529-013-1226-2
PubMed: 23690038
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Stress, Physiological (MeSH)</term><term>Superoxide Dismutase (analysis)</term><term>Tobacco (enzymology)</term><term>Tobacco (genetics)</term><term>Tobacco (radiation effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Basse température (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Feuilles de plante (composition chimique)</term><term>Glucose 6-phosphate dehydrogenase (génétique)</term><term>Glucose 6-phosphate dehydrogenase (métabolisme)</term><term>Malonaldéhyde (analyse)</term><term>Peroxydes (analyse)</term><term>Phylogenèse (MeSH)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Stress physiologique (MeSH)</term><term>Superoxide dismutase 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xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glucose 6-phosphate dehydrogenase</term><term>Populus</term><term>Protéines recombinantes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucose 6-phosphate dehydrogenase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="radiation effects" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cold Temperature</term><term>Gene Expression</term><term>Gene Expression Profiling</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Sequence Alignment</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de profil d'expression de gènes</term><term>Basse température</term><term>Expression des gènes</term><term>Phylogenèse</term><term>Stress physiologique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23690038</PMID><DateCompleted><Year>2014</Year><Month>02</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>08</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>35</Volume><Issue>9</Issue><PubDate><Year>2013</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.</ArticleTitle><Pagination><MedlinePgn>1509-18</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-013-1226-2</ELocationID><Abstract><AbstractText>A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Yuanzhen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Forestry, South China Agricultural University, Guangzhou, 510642, China. yzhlin@scau.edu.cn</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Shanzhi</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Hai</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Zhiyi</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xiaoyang</ForeName><Initials>X</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010545">Peroxides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>4Y8F71G49Q</RegistryNumber><NameOfSubstance UI="D008315">Malondialdehyde</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.1.49</RegistryNumber><NameOfSubstance UI="D005954">Glucosephosphate Dehydrogenase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.15.1.1</RegistryNumber><NameOfSubstance UI="D013482">Superoxide 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MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence 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PubStatus="entrez"><Year>2013</Year><Month>5</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>5</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>2</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23690038</ArticleId><ArticleId IdType="doi">10.1007/s10529-013-1226-2</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Xiaoyang" sort="Chen, Xiaoyang" uniqKey="Chen X" first="Xiaoyang" last="Chen">Xiaoyang Chen</name><name sortKey="Guo, Hai" sort="Guo, Hai" uniqKey="Guo H" first="Hai" last="Guo">Hai Guo</name><name sortKey="Lin, Shanzhi" sort="Lin, Shanzhi" uniqKey="Lin S" first="Shanzhi" last="Lin">Shanzhi Lin</name><name sortKey="Zhang, Zhiyi" sort="Zhang, Zhiyi" uniqKey="Zhang Z" first="Zhiyi" last="Zhang">Zhiyi Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Yuanzhen" sort="Lin, Yuanzhen" uniqKey="Lin Y" first="Yuanzhen" last="Lin">Yuanzhen Lin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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